Diacetylchitobiose deacetylase
WebJan 15, 2016 · N,N'-diacetylchitobiose deacetylase (Dac) belongs to the CE-14 family and plays a role in the chitinolytic pathway in archaea by deacetylating N,N'-diacetylchitobiose (GlcNAc2), which is the end product of chitinase ... Enzymes of carbohydrate esterase (CE) family 14 catalyze hydrolysis of N-acetyl groups at the non-reducing end of the N ... WebThe hyperthermophilic archaeon Thermococcus kodakaraensis KOD1 possesses chitinase (Tk-ChiA) and exo-β-d-glucosaminidase (Tk-GlmA) for chitin degradation; the former produces diacetylchitobiose (GlcNAc2) from chitin, and the latter hydrolyzes chitobiose (GlcN2) to glucosamine (GlcN). To identify the enzyme that physiologically …
Diacetylchitobiose deacetylase
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Webdiacetylchitobiose deacetylase (Dac), which was firstly derived from hyperthermophilic bacteria, to demonstrate the advantages of our method. With the optimized fabrication steps, our hydrogel WebOct 28, 2024 · Deacetylase from the extremophile Pyrococcus chitonophagus has been analyzed by X-ray crystallography, small-angle X-ray scattering, differential scanning calorimetry, isothermal titration calorimetry and NMR to determine its structure, thermodynamics and enzymatic properties ...
WebOct 28, 2024 · Deacetylase from the extremophile Pyrococcus chitonophagus has been analyzed by X-ray crystallography, small-angle X-ray scattering, differential scanning … WebAug 14, 2024 · Diacetylchitobiose deacetylase has great application potential in the production of chitosan oligosaccharides and monosaccharides. This work aimed to …
WebApr 28, 2014 · Concerted action of diacetylchitobiose deacetylase and exo-beta-D-glucosaminidase in a novel chitinolytic pathway in the hyperthermophilic archaeon Thermococcus kodakaraensis KOD1. Tanaka T , Fukui T , Fujiwara S , Atomi H , Imanaka T WebJul 16, 2004 · The hyperthermophilic archaeon Thermococcus kodakaraensis KOD1 possesses chitinase (Tk-ChiA) and exo-β-d-glucosaminidase (Tk-GlmA) for chitin degradation; the former produces diacetylchitobiose (GlcNAc 2) from chitin, and the latter hydrolyzes chitobiose (GlcN 2) to glucosamine (GlcN).To identify the enzyme that …
WeblationofGlcNActoformGlcN,diacetylchitobiose(or chitin disaccharide) deacetylase, and N-acetyl-D-glucosamine-6-phosphate (GlcNAc-6P) deacetylase. Diacetylchitobiosedeacetylase(EC3.5.1.105)belongs to the carbohydrate esterase family 14, it is the key enzyme that catalyzes the hydrolysis of N-acetyl groups at the non …
WebJan 24, 2024 · Diacetylchitobiose deacetylase (EC 3.5.1.105) belongs to the carbohydrate esterase family 14, it is the key enzyme that catalyzes the hydrolysis of N-acetyl groups … chinese food dundee ilWebDec 1, 2024 · Diacetylchitobiose deacetylase (Dac) from Pyrococcus horikoshii can realize the one-step production of glucosamine (GlcN). The efficient expression and secretion of Dac play a central role in the green production of GlcN. In this study, Bacillus subtilis WB600 was used as the expression host. Firstly, we screened 12 signal … grand isle grand havenWebSelect search scope, currently: articles+ all catalog, articles, website, & more in one search; catalog books, media & more in the Stanford Libraries' collections; articles+ journal articles & other e-resources chinese food dunn ncWebOct 1, 2024 · Directed evolution of diacetylchitobiose deacetylase via high-throughput droplet sorting with a novel, bacteria-based biosensor Request PDF Directed evolution … grand isle high school laWebJan 11, 2024 · For example, PhDac, a diacetylchitobiose deacetylase with high catalytic activity for GlcNAc monomer and dimer, was heterologously expressed in B. subtilis by Jiang et al. for the green production of GlcN. However, the optimal pH and temperature of PhDac was not suitable for the stability of GlcN. grand isle historical societyWebConcerted Action of Diacetylchitobiose Deacetylase and Exo- -D-glucosaminidase in a Novel Chitinolytic Pathway in the Hyperthermophilic Archaeon Thermococcus kodakaraensis KOD1* Received for publication, December 26, 2003, and in revised form, May 3, 2004 Published, JBC Papers in Press, May 10, 2004, DOI 10.1074/jbc.M314187200 chinese food dupont circleWebJul 1, 2013 · The purified Pyrococcus Dacs were active and thermostable up to 85 °C. The crystal structures of Ph-Dac and Pf-Dac were determined at resolutions of 2.0 Å and 1.54 Å, respectively. The Pyrococcus Dac forms a hexamer composed of two trimers. These Dacs are characterized by an intermolecular cleft, which is formed by two polypeptides in the ... chinese food dyersburg tn